* Test available on a research basis only. Contact ISI for details.
* Test available on a research basis only. Contact ISI for details.
* Test available on a research basis only. Contact ISI for details.
* Test available on a research basis only. Contact ISI for details.
Clinical Significance:
The Interleukins belong to the family termed cytokines. They are peptides used by immune and inflammatory cells to communicate and control cell operations. The cytokines have some similar actions to the Growth Factors but Growth Factors regulate proliferation of non-immune cells. Interleukin 1a is a 17,500 molecular weight peptide derived primarily from macrophages, fibroblasts, endothelial cells, and B cells. The major target cells are T and B cells, Fibroblasts, and Hepatocytes. Interleukin 1a shares a receptor with Interleukin 1b although they are significantly different structurally. Interleukin 1a promotes antigen specific immune responses, inflammation, Prostaglandin secretion, Colony Stimulating Factors, proteoglycanase, collagenase, and gelatinase activity, and release of Interleukin 2 from T cells. Levels are stimulated by liposaccharide, endotoxins, inflammatory agents, lectin, Tumor Necrosis Factor, and Interferons. Levels are suppressed by Corticosteroids, Prostaglandin E2, and suppressant lymphocytes.
Reference Range:
Reference Range available by report.
Procedure:
Interleukin 1a is measured by direct radioimmunoassay/enzyme immunoassay.
Patient Preparation:
Patient should not be on any Corticosteroids, anti-inflammatory medications or pain killers, if possible, for at least 48 hours prior to collection of specimen.
Specimen Collection:
3 ml serum or EDTA plasma should be collected and separated as soon as possible. Freeze specimen immediately after separation. Minimum specimen size is 1 ml.
Special Specimens:
For tumor/tissue and various fluids (i.e. CSF, peritoneal, synovial, etc.) contact the Institute for requirements and special handling.
Shipping Instructions:
Ship specimens frozen in dry ice.
References:
1. JT Whicher and SW Evans. Cytokines in Disease. Clinical Chemistry 36: 1269-1281, 1990.
2. MP Bevilacqua, JS Pober, GR Majeau, W Fiers, RS Cotran, and MA Gimbrone. Recombinant Tumor Necrosis Factor Induced Pro-Coagulant Activity in Cultured Human Vascular Endothelium: Characterization and Comparison with Action of Interleukin-1. Proceedings of the National Academy of Science 83: 4533-4537, 1986.
Clinical Significance:
The Interleukins belong to the family termed cytokines. They are peptides used by immune and inflammatory cells to communicate and control cell operations. The cytokines have some similar actions to the Growth Factors but Growth Factors regulate proliferation of non-immune cells. Interleukin 1b is a 17,500 molecular weight peptide derived primarily from macrophages, fibroblasts, endothelial cells, and B cells. The major target cells are T and B cells, Fibroblasts, and Hepatocytes and it has pyrogenic activity. Interleukin 1b shares a receptor with Interleukin 1a although they are significantly different structurally. Interleukin 1b promotes antigen specific immune responses, inflammation, secretion, Colony Stimulating Factors, proteoglycanase, collagenase, and gelatinase activity, acute phase response, and cartilage resorption. Interleukin 1b increases accumulation of cell-associated and extracellular arachadonic acid, and induces release of Interleukin 6.
Reference Range:
Reference Range available by report.
Procedure:
Interleukin 1b is measured by direct radioimmunoassay/enzyme immunoassay.
Patient Preparation:
Patient should not be on any Corticosteroids, anti-inflammatory medications or pain killers, if possible, for at least 48 hours prior to collection of specimen.
Specimen Collection:
3 ml serum or EDTA plasma should be collected and separated as soon as possible. Freeze specimen immediately after separation. Minimum specimen size is 1 ml.
Special Specimens:
For tumor/tissue and various fluids (i.e. CSF, peritoneal, synovial, etc.) contact the Institute for requirements and special handling.
Shipping Instructions:
Ship specimens frozen in dry ice.
References:
1. JT Whicher and SW Evans. Cytokines in Disease. Clinical Chemistry 36: 1269-1281, 1990.
2. MP Bevilacqua, JS Pober, GR Majeau, W Fiers, RS Cotran, and MA Gimbrone. Recombinant Tumor Necrosis Factor Induced Pro-Coagulant Activity in Cultured Human Vascular Endothelium: Characterization and Comparison with Action of Interleukin-1. Proceedings of the National Academy of Science 83: 4533-4537, 1986.
Clinical Significance:
The Interleukins belong to the family termed cytokines. They are peptides used by immune and inflammatory cells to communicate and control cell operations. The cytokines have some similar actions to the Growth Factors but Growth Factors regulate proliferation of non-immune cells. Interleukin 2 is a 15,000 molecular weight glycoprotein originally called T Cell Growth Factor. It is released primarily by activated T Cells. Its target cells include T and B cells, Natural Killer Cells, and Lymphokine-activated Killer Cells. Interleukin 2 increases alloantigen responses and improves recovery of immune function. It also induces production of other lymphokines including Tumor Necrosis Factor, g Interferon, and Interleukin 6. It is bound to specific Interleukin 2 receptors located on activated T cells, resting and activated B cells, macrophages, and mast cells.
Reference Range:
Reference Range available by report.
Procedure:
Interleukin 2 is measured by direct radioimmunoassay/enzyme immunoassay.
Patient Preparation:
Patient should not be on any Corticosteroids, anti-inflammatory medications or pain killers, if possible, for at least 48 hours prior to collection of specimen.
Specimen Collection:
3 ml serum or EDTA plasma should be collected and separated as soon as possible. Freeze specimen immediately after separation. Minimum specimen size is 1 ml.
Special Specimens:
For tumor/tissue and various fluids (i.e. CSF, peritoneal, synovial, etc.) contact the Institute for requirements and special handling.
Shipping Instructions:
Ship specimens frozen in dry ice.
References:
1. JT Whicher and SW Evans. Cytokines in Disease. Clinical Chemistry 36: 1269-1281, 1990.
2. CM Huang, RJ Elin, M Ruddel, C Silva, MT Lotze, and SA Rosenberg. Changes in Laboratory Results for Cancer Patients Treated with Interleukin-2. Clinical Chemistry 36: 431-434, 1990.
Clinical Significance:
The Interleukins belong to the family termed cytokines. They are peptides used by immune and inflammatory cells to communicate and control cell operations. The cytokines have some similar actions to the Growth Factors but Growth Factors regulate proliferation of non-immune cells. Interleukin 3 is a 20,000 - 26,000 molecular weight glycoprotein originally called Multi-Colony Stimulating Factor. It is released primarily by activated T Cells. Its primary target cells are early hemopoietic cells. Interleukin 3 also stimulates proliferation of macrophages, neutrophils, eosinophils, megakaryocytes, and mast cells. Interleukin 3 activates pluripotent stem cells giving rise to a wide range of cell types. It also induces 20a-Steroid Dehydrogenase in T cells and Leukotriene C4. It works in conjunction with Interleukin 6 to hasten the emergence of blast cell colony-forming cells.
Reference Range:
Reference Range available by report.
Procedure:
Interleukin 3 is measured by direct radioimmunoassay/enzyme immunoassay.
Patient Preparation:
Patient should not be on any Corticosteroids, anti-inflammatory medications or pain killers, if possible, for at least 48 hours prior to collection of specimen.
Specimen Collection:
3 ml serum or EDTA plasma should be collected and separated as soon as possible. Freeze specimen immediately after separation. Minimum specimen size is 1 ml.
Special Specimens:
For tumor/tissue and various fluids (i.e. CSF, peritoneal, synovial, etc.) contact the Institute for requirements and special handling.
Shipping Instructions:
Ship specimens frozen in dry ice.
References:
1. JT Whicher and SW Evans. Cytokines in Disease. Clinical Chemistry 36: 1269-1281, 1990.
2. JN Ihle. The Molecular and Cellular Biology of Interleukin-3. Yearbook of Immunology 5: 59-102, 1989.
Clinical Significance:
The Interleukins belong to the family termed cytokines. They are peptides used by immune and inflammatory cells to communicate and control cell operations. The cytokines have some similar actions to the Growth Factors but Growth Factors regulate proliferation of non-immune cells. Interleukin 4 is a 20,000 molecular weight glycoprotein originally called T Cell Growth Factor along with Interleukin 2. It is released primarily by activated T Cells and mast cells. Its primary target cells are T and B cells, macrophage/monocytes, and mast cells. Interleukin 4 activates B cell differentiation and immunoglobulins prior to action of Interleukin 6. It is also a co-stimulator along with anti-immunoglobulin antibodies of B cell proliferation. Interleukin 4 is bound to receptors located on activated T cells, resting and activated B cells, macrophages, and mast cells.
Reference Range:
Reference Range available by report.
Procedure:
Interleukin 4 is measured by direct radioimmunoassay/enzyme immunoassay.
Patient Preparation:
Patient should not be on any Corticosteroids, anti-inflammatory medications or pain killers, if possible, for at least 48 hours prior to collection of specimen.
Specimen Collection:
3 ml serum or EDTA plasma should be collected and separated as soon as possible. Freeze specimen immediately after separation. Minimum specimen size is 1 ml.
Special Specimens:
For tumor/tissue and various fluids (i.e. CSF, peritoneal, synovial, etc.) contact the Institute for requirements and special handling.
Shipping Instructions:
Ship specimens frozen in dry ice.
References:
1. JT Whicher and SW Evans. Cytokines in Disease. Clinical Chemistry 36: 1269-1281, 1990.
2. JP Galizzi, B Castle, and O Djossou. Purification of a 130K Da T-cell Glycoprotein that Binds Human Interleukin-4 with High Affinity. Journal of Biological Chemistry 265: 439-44, 1990.
Clinical Significance:
The Interleukins belong to the family termed cytokines. They are peptides used by immune and inflammatory cells to communicate and control cell operations. The cytokines have some similar actions to the Growth Factors but Growth Factors regulate proliferation of non-immune cells. Interleukin 5 is a 50,000-60,000 molecular weight glycoprotein originally called T Cell Replacing Factor. It is released primarily by activated T Cells. Its primary target cells are B cells and eosinophils. Interleukin 5 activates B cell differentiation. Its actions are similar to Interleukin 3. Interleukin 5 primes basophils for agonist-induced generation of Leukotriene C4. It is stimulated by Interleukin 2. Interleukin 5 stimulates growth and differentiation of eosinophil progenitor without influencing cells committed to neutrophils, basophils, or monocytes. It enhances responsiveness of eosinophils inducing polarization, shape change, chemotaxis, cytotoxity, oxygen radical release and prolongs survival.
Reference Range:
Reference Range available by report.
Procedure:
Interleukin 5 is measured by direct radioimmunoassay/enzyme immunoassay.
Patient Preparation:
Patient should not be on any Corticosteroids, anti-inflammatory medications or pain killers, if possible, for at least 48 hours prior to collection of specimen.
Specimen Collection:
3 ml serum or EDTA plasma should be collected and separated as soon as possible. Freeze specimen immediately after separation. Minimum specimen size is 1 ml.
Special Specimens:
For tumor/tissue and various fluids (i.e. CSF, peritoneal, synovial, etc.) contact the Institute for requirements and special handling.
Shipping Instructions:
Ship specimens frozen in dry ice.
References:
1. JT Whicher and SW Evans. Cytokines in Disease. Clinical Chemistry 36: 1269-1281, 1990.
2. SC Bischoff, T Brunner, AL DeWeck, and LA Dahinden. Interleukin-5 Modifies Histamine Release and Leukotriene Generation by Human Basophils in Response to Diverse Agonists. Journal of Experimental Medicine 18: 1577-1582, 1990.
Clinical Significance:
The Interleukins belong to the family termed cytokines. They are peptides used by immune and inflammatory cells to communicate and control cell operations. The cytokines have some similar actions to the Growth Factors but Growth Factors regulate proliferation of non-immune cells. Interleukin 6 is a 25,000 molecular weight glycoprotein produced primarily in macrophages, T cells, fibroblasts and endothelial cells. Its primary target cells are T and B cells and neutrophils. Its main actions are involvement in terminal differentiation of B cells to antibody secreting plasma cells, activation of T cells and stimulation of hepatocyte production of acute phase proteins. Interleukin stimulates Prolactin, Growth Hormone, Luteinizing Hormone, Follicle Stimulating Hormone and ACTH. Release is stimulated by Interleukin 1a and b, Vasoactive Intestinal Polypeptide, and Prostaglandin E2. Release is not affected by aspirin or indomethacin. Levels are elevated in multiple myeloma, rheumatoid arthritis, systemic lupus erythematosus, meningococcus meningitis and infectious peritonitis.
Reference Range:
Reference Range available by report.
Procedure:
Interleukin 6 is measured by direct radioimmunoassay/enzyme immunoassay.
Patient Preparation:
Patient should not be on any Corticosteroids, anti-inflammatory medications or pain killers, if possible, for at least 48 hours prior to collection of specimen.
Specimen Collection:
3 ml serum or EDTA plasma should be collected and separated as soon as possible. Freeze specimen immediately after separation. Minimum specimen size is 1 ml.
Special Specimens:
For tumor/tissue and various fluids (i.e. CSF, peritoneal, synovial, etc.) contact the Institute for requirements and special handling.
Shipping Instructions:
Ship specimens frozen in dry ice.
References:
1. JT Whicher and SW Evans. Cytokines in Disease. Clinical Chemistry 36: 1269-1281, 1990.
2. BL Spangelo, WD Jarvis, AM Judd, and RM MacLeod. Induction of Interleukin-6 Release by Interleukin-1 in Rat Anterior Pituitary Cells in vitro: Evidence for an Eicosanoid Dependent Mechanism. Endocrinology 129: 2886-2894, 1991.
Clinical Significance:
The Interleukins belong to the family termed cytokines. They are peptides used by immune and inflammatory cells to communicate and control cell operations. The cytokines have some similar actions to the Growth Factors but Growth Factors regulate proliferation of non-immune cells. Interleukin 7 is a 25,000 molecular weight glycoprotein produced primarily in bone marrow stromal cells. Its primary target cells are B cells. Interleukin 7 supports long term culture of B cells. Interleukin 7 plays an important role in maintenance of a population of pre B lymphocytes although it does not stimulate differention of pre B cells to B cells. Interleukin 7 also activates myeloid cell proliferation and differentiation.
Reference Range:
Reference Range available by report.
Procedure:
Interleukin 7 is measured by direct radioimmunoassay/enzyme immunoassay.
Patient Preparation:
Patient should not be on any Corticosteroids, anti-inflammatory medications or pain killers, if possible, for at least 48 hours prior to collection of specimen.
Specimen Collection:
3 ml serum or EDTA plasma should be collected and separated as soon as possible. Freeze specimen immediately after separation. Minimum specimen size is 1 ml.
Special Specimens:
For tumor/tissue and various fluids (i.e. CSF, peritoneal, synovial, etc.) contact the Institute for requirements and special handling.
Shipping Instructions:
Ship specimens frozen in dry ice.
References:
1. JT Whicher and SW Evans. Cytokines in Disease. Clinical Chemistry 36: 1269-1281, 1990.
2. P Hunt, D Robertson, D Weiss, D Rennick, F Lee, and ON Witte. A Single Marrow-Derived Stromal Cell Type Supports the in vitro Growth of Early Lymphoid and Myeloid Cells. Cell 48: 997-1007, 1987.
Clinical Significance:
The Interleukins belong to the family termed cytokines. They are peptides used by immune and inflammatory cells to communicate and control cell operations. The cytokines have some similar actions to the Growth Factors but Growth Factors regulate proliferation of non-immune cells. Interleukin 8 is a 6,000 - 8000 molecular weight glycoprotein produced primarily by macrophages and monocytes. Its primary target cells are neutrophils. Interleukin 8 is a chemotactic agent for monocytes and neutrophils. Interleukin 8 causes oxygen radical and enzyme release by neutrophils and mobilizes products into circulation. Interleukin 8 is stimulated by Interleukin 1 and Tumor Necrosis Factor.
Reference Range:
Reference Range available by report.
Procedure:
Interleukin 8 is measured by direct radioimmunoassay/enzyme immunoassay.
Patient Preparation:
Patient should not be on any Corticosteroids, anti-inflammatory medications or pain killers, if possible, for at least 48 hours prior to collection of specimen.
Specimen Collection:
3 ml serum or EDTA plasma should be collected and separated as soon as possible. Freeze specimen immediately after separation. Minimum specimen size is 1 ml.
Special Specimens:
For tumor/tissue and various fluids (i.e. CSF, peritoneal, synovial, etc.) contact the Institute for requirements and special handling.
Shipping Instructions:
Ship specimens frozen in dry ice.
References:
1. JT Whicher and SW Evans. Cytokines in Disease. Clinical Chemistry 36: 1269-1281, 1990.
2. K Matsushima, JJ Oppenheim. Interleukin-8 and Macrophage Colony Stimulating Factor: Novel Inflammatory Cytokines Inducible by Interleukin I and Tumor Necrosis Factor. Cytokine 1: 2 - 13, 1989.
Clinical Significance:
The Interleukins belong to the family termed cytokines. They are peptides used by immune and inflammatory cells to communicate and control cell operations. The cytokines have some similar actions to the Growth Factors but Growth Factors regulate proliferation of non-immune cells.
Reference Range:
Reference Range available by report.
Procedure:
Interleukin 10 is measured by direct radioimmunoassay/enzyme immunoassay.
Patient Preparation:
Patient should not be on any Corticosteroids, anti-inflammatory medications or pain killers, if possible, for at least 48 hours prior to collection of specimen.
Specimen Collection:
3 ml serum or EDTA plasma should be collected and separated as soon as possible. Freeze specimen immediately after separation. Minimum specimen size is 1 ml.
Special Specimens:
For tumor/tissue and various fluids (i.e. CSF, peritoneal, synovial, etc.) contact the Institute for requirements and special handling.
Shipping Instructions:
Ship specimens frozen in dry ice.
References:
1. JT Whicher and SW Evans. Cytokines in Disease. Clinical Chemistry 36: 1269-1281, 1990.
2. K Matsushima, JJ Oppenheim. Interleukin-8 and Macrophage Colony Stimulating Factor: Novel Inflammatory Cytokines Inducible by Interleukin I and Tumor Necrosis Factor. Cytokine 1: 2 - 13, 1989.
Clinical Significance:
The Interleukins belong to the family termed cytokines. They are peptides used by immune and inflammatory cells to communicate and control cell operations. The cytokines have some similar actions to the Growth Factors but Growth Factors regulate proliferation of non-immune cells.
Reference Range:
Reference Range available by report.
Procedure:
Interleukin 12 is measured by direct radioimmunoassay/enzyme immunoassay.
Patient Preparation:
Patient should not be on any Corticosteroids, anti-inflammatory medications or pain killers, if possible, for at least 48 hours prior to collection of specimen.
Specimen Collection:
3 ml serum or EDTA plasma should be collected and separated as soon as possible. Freeze specimen immediately after separation. Minimum specimen size is 1 ml.
Special Specimens:
For tumor/tissue and various fluids (i.e. CSF, peritoneal, synovial, etc.) contact the Institute for requirements and special handling.
Shipping Instructions:
Ship specimens frozen in dry ice.
References:
1. JT Whicher and SW Evans. Cytokines in Disease. Clinical Chemistry 36: 1269-1281, 1990.
2. K Matsushima, JJ Oppenheim. Interleukin-8 and Macrophage Colony Stimulating Factor: Novel Inflammatory Cytokines Inducible by Interleukin I and Tumor Necrosis Factor. Cytokine 1: 2 - 13, 1989.
Clinical Significance:
The Interleukins belong to the family termed cytokines. They are peptides used by immune and inflammatory cells to communicate and control cell operations. The cytokines have some similar actions to the Growth Factors but Growth Factors regulate proliferation of non-immune cells.
Reference Range:
Reference Range available by report.
Procedure:
Interleukin 13 is measured by direct radioimmunoassay/enzyme immunoassay.
Patient Preparation:
Patient should not be on any Corticosteroids, anti-inflammatory medications or pain killers, if possible, for at least 48 hours prior to collection of specimen.
Specimen Collection:
3 ml serum or EDTA plasma should be collected and separated as soon as possible. Freeze specimen immediately after separation. Minimum specimen size is 1 ml.
Special Specimens:
For tumor/tissue and various fluids (i.e. CSF, peritoneal, synovial, etc.) contact the Institute for requirements and special handling.
Shipping Instructions:
Ship specimens frozen in dry ice.
References:
1. JT Whicher and SW Evans. Cytokines in Disease. Clinical Chemistry 36: 1269-1281, 1990.
2. K Matsushima, JJ Oppenheim. Interleukin-8 and Macrophage Colony Stimulating Factor: Novel Inflammatory Cytokines Inducible by Interleukin I and Tumor Necrosis Factor. Cytokine 1: 2 - 13, 1989.
* Test available on a research basis only. Contact ISI for details.
* Test available on a research basis only. Contact ISI for details.
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