17-Hydroxy Progesterone, 24-Hour Urine

• ISI Order Code:

  17OHP 24HR

• CPT Code:

  83498

Clinical Significance:

17-Hydroxy Progesterone is a steroid derived primarily from enzymatic metabolism of Progesterone and 17-Hydroxy Pregnenolone. It is converted enzymatically to Androstenedione and 11-Deoxycortisol. It is produced in both the gonads and adrenal glands. It is excreted into the urine in conjugated and unconjugated forms of 17-Hydroxy Progesterone and as Pregnanetriol. This assay measures the total of the conjugated and unconjugated forms. It is stimulated by ACTH and suppressed by Dexamethasone. Levels of urine 17-Hydroxy Progesterone are greatly increased in patients with Polycystic Ovarian Disease and Congenital Adrenal Hyperplasia and show exaggerated responses to ACTH in these cases. 17-Hydroxy Progesterone is the marker steroid for determining cases of 21a-Hydroxylase Deficient Congenital Adrenal Hyperplasia. Urine levels are frequently elevated in patients with idiopathic hirsutism

• Reference Range:

  Urine Adults

  • Male:

    Up to 2.0 µg/24 hrs.

  • Female:

    Up to 4.5 µg/24 hrs.

  Pediatric Ranges:

  • Newborns & Infants:

    3 Days to 1 Year: Up to 50 ng/24 hrs.

  Children:

  • 1–8 Years: Up to 300 ng/24 hrs.

• Procedure:

  Urine 17-Hydroxy Progesterone is measured by an immunoassay.

• Expected Turnaround Time:

  7 Business Days

Turnaround time is defined as the usual number of days from the date of receipt of a specimen for testing to when the result is released to the ordering facility.

• Report:

  Sample Report

Patient Preparation:

Patient should not be on any Corticosteroid, ACTH, Estrogen, or Gonadotropin medications, if possible, for at least 48 hours prior to collection of specimen.

Specimen Collection:

24 hours urine collection. Refrigerate during collection and mix well. Store specimen refrigerated during collection. No preservatives are required. Transfer 5 – 10 ml urine to urine transport container and freeze immediately. Specimens should be frozen prior to shipping.
Minimum urine specimen size is 1.0 mL

Shipping Instructions:

Ship specimens frozen in dry ice.

References:

1.E Carmina and RA Lobo.  Pituitary-Adrenal Responses to Corticotropin-Releasing
Factor in Late Onset 21-Hydroxylase Deficiency.  Fertility and Sterility 54: 79-83, 1990.

2.ET Wong, DR Brown, RA Ulstrom, and MW Steffes.  Urinary 17-alpha-
Hydroxyprogesterone in Diagnosis and Management of Congenital Adrenal
Hyperplasia.  Journal of Clinical Endocrinology and Metabolism 49: 377, 1979.