ISI Order Code:
ANG2
CPT Code:
82163
Clinical Significance
Angiotensin II is an eight amino acid peptide formed by Angiotensin Converting Enzyme (ACE) cleavage of Angiotensin I. Angiotensin II is metabolized further to Angiotensin III. Angiotensin II release is controlled by Renin, blood pressure, blood volume, sodium balance and by Aldosterone concentration. Levels of Angiotensin II are increased in many types of hypertension. Angiotensin II stimulates the release of Anti-Diuretic Hormone, ACTH, Prolactin, Luteinizing Hormone, Oxytocin and Aldosterone. Angiotensin II increases vasoconstriction and inhibits tubular resorption of sodium, and can increase endothelial cell growth.
Reference Range:
10 – 60 pg/ml.
Procedure:
Angiotensin II is measured by direct ELISA.
Expected Turnaround Time:
7-10 Business Days
Report:
Turnaround time is defined as the usual number of days from the date of receipt of a specimen for testing to when the result is released to the ordering facility.
Patient Preparation
Patient should be on a normal sodium diet, 110 mEq. sodium. Patient should be in a recumbent posture for at least 30 minutes prior to collection of specimen. Diuretics, mineralocorticoids, glucocorticoids, estrogens, oral contraceptives, and ACTH medications and sodium, potassium, and posture all affect Angiotensin levels.
Specimen Collection
3 ml EDTA plasma should be collected and separated as soon as possible. Freeze plasma immediately after separation. Minimum specimen size is 1 ml.
Rejection Criteria:
Gross hemolysis/lipemia/icteric
Special Specimens
For tumor/tissue and various fluids (i.e. CSF, peritoneal, synovial, etc.), contact the Institute for requirements and special handling.
Shipping Instructions
Ship specimens frozen in dry ice.
References
1. van Hooft IMS, Grobbee DE, Derkx FHM, et al. Renal Hemodynamics and the Renin-Angiotensin-Aldosterone System in Normotensive Subjects with Hypertensive and Normotensive Patients. N Engl J Med 324:1305-1311, 1991.
2. Kosunin KJ and Pakarinen A. Correlations Between Plasma Renin Activity, Angiotensin II, and Aldosterone. J Clin Endocrinol Metab. 47:665,1978.